Journal: Frontiers in Oncology

Article Title: High-Content Monitoring of Drug Effects in a 3D Spheroid Model

doi: 10.3389/fonc.2017.00293

Figure Lengend Snippet: Commercial microtiter plates for 3D spheroid assays.

Article Snippet: CellCarrier ® ULA , 6055330, 6055334 , 96 , U , c , NAC , PerkinElmer.

Techniques:

Journal: Cell Reports Methods

Article Title: Image-based and machine learning-guided multiplexed serology test for SARS-CoV-2

doi: 10.1016/j.crmeth.2023.100565

Figure Lengend Snippet:

Article Snippet: In the automated assay, the transfected cells were seeded to CellCarrier Ultra 384-well microplates (PerkinElmer, US) with a seeding density of approximately 3–4 x 10ˆ3 cells/well in 25 μL per well using MultiFlo FX RAD dispenser (BioTek, US) and incubated at 37°C humidified atmosphere for 48 h in the growth media.

Techniques: Recombinant, Membrane, Expressing, Plasmid Preparation, Clone Assay, Software, Cell Culture, Imaging, Microscopy, High Content Screening, Transferring

Journal: Scientific Reports

Article Title: Acquisition of fluoroquinolone resistance leads to increased biofilm formation and pathogenicity in Campylobacter jejuni

doi: 10.1038/s41598-019-54620-1

Figure Lengend Snippet: ( A ) 72 h biofilm assays were carried out on Cellcarrier Ultra 96 well plates followed by staining of viable C . jejuni with the vital stain TAMRA for 30 minutes. For NCTC11168 a stark increase in the formation of a mature, viable biofilm is present in aerobic conditions (AER) but not microaerobic conditions (MA) when a relaxed DNA supercoiling phenotype is induced by growth in the presence of 10μg/ml Novobiocin. Data is representative of three biological replicates. ( B ) Confocal slices of TAMRA (live bacteria, red) and SytoX (DNA, green) stained induced biofilm after 72 h incubation in aerobic conditions. Relaxation of DNA supercoiling results in an increase in both viable NCTC 11168 as well as fibers of eDNA, indicating a mature C . jejuni biofilm (scale bar = 10 μm).

Article Snippet: Overnight cultures were equalised to an OD600 of 0.1 in MH broth and 200 μl was seeded in triplicate into the wells of a PerkinElmer CellCarrier ultra optical 96 well plate.

Techniques: Staining, Incubation